Debuting the first service of its kind in Malaysia, Arcadia’s targeted protein analysis service, leverages on Olink Proteomics® Proximity Extension Assay (PEA) technology, which enables detection and quantification of circulating proteins, providing an in-depth, biological understanding of disease with high specificity and sensitivity using minimal sample amounts. This service output is for research use only (RUO).
For the detection, a matched pair of antibodies labeled with complementary DNA oligonucleotides binds to the respective protein target and comes in proximity, resulting in the hybridization of oligonucleotides labels. The formed double-stranded product is then amplified by PCR and can be detected by a NGS or qPCR.
Targeted Panels | Olink® Target 48 Cytokines (T48) | Olink® Target 96 (T96) | |
Targeted Proteins | 45 proteins | 92 proteins | |
Panels | 1 Panel for a comprehensive study of inflammation in absolute quantification. | 14 panels grouped by diseases areas and biological processes | |
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Panel Selection | Sample Type | Tube Type | Minimum amount per sample | Minimum Sample Number |
Target 48
& Target 96 |
Plasma (Collected from EDTA blood tube only) |
Leak proof sample tube, recommended protein-lobind tube | 30µL | Olink® is a high-throughput application, so we ask clients to provide enough samples to fill a plate (minimum 88 samples for Target 96 and 40 samples for Target 48) to ensure statistical significance. |
Serum | Leak proof sample tube, recommended protein-lobind tube | 30µL | ||
Whole Blood | Red top
SST EDTA |
1 – 5 mL |
LCMS targeted assay |
Tool for novel biomarker target Post-translational ready for lipidomics, glycomics, metabolomics studies. |
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