WHAT WE CAN DOProtein Analysis & Algorithm

Arcadia offers cutting-edge proteomics analyses, seamlessly integrated with the prowess of artificial intelligence, designed to unlock the answers to your biological inquiries.
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SERVICESTargeted Protein Analysis (PEA)

Debuting the first service of its kind in Malaysia, Arcadia’s targeted protein analysis service, leverages on Olink Proteomics® Proximity Extension Assay (PEA) technology, which enables detection and quantification of circulating proteins, providing an in-depth, biological understanding of disease with high specificity and sensitivity using minimal sample amounts. This service output is for research use only (RUO).

How does it work?

For the detection, a matched pair of antibodies labeled with complementary DNA oligonucleotides binds to the respective protein target and comes in proximity, resulting in the hybridization of oligonucleotides labels. The formed double-stranded product is then amplified by PCR and can be detected by a NGS or qPCR.

SERVICESTargeted Panels Available

Targeted Panels           Olink® Target 48 Cytokines (T48) Olink® Target 96 (T96)
Targeted Proteins 45 proteins 92 proteins
Panels 1 Panel for a comprehensive study of inflammation in absolute quantification. 14 panels grouped by diseases areas and biological processes
  • Olink® Target 96 Inflammation
  • Olink®  Target 96 Immuno-Oncology
  • Olink® Target 96 Immune Response
  • Olink® Target 96 Organ Damage
  • Olink® Target 96 Metabolism
  • Olink® Target 96 Development
  • Olink® Target 96 Cardiometabolic
  • Olink® Target 96 Cell Regulation
  • Olink® Target 96 Neuro Exploratory
  • Olink® Target 96 Oncology III
  • Olink® Target 96 Cardiovascular II
  • Olink® Target 96 Cardiovascular III
  • Olink® Target 96 Oncology II
  • Olink® Target 96 Neurology
Please contact us for consultation and further details.

SERVICESTargeted Protein Analysis (PEA) Service Flow

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Customer's Inquiry
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Quotation Creation
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Sample Submission
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Protein Analysis
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Statistical Analysis
Please contact us for consultation and further details.

SERVICESTargeted Protein Analysis (PEA) Deliverables

  • Absolute quantification in pg/ml (Target 48 only)
  • Data in Normalized Protein Expression (NPX) format for relative quantification (Target 96)
  • Certificate of Analysis showing quality details – internal and external controls are included throughout the workflow to ensure data quality
  • Data visualization :
    • Hypothesis testing
    • Volcano
    • Boxplot
    • Heatmap
    • PCA plot
  • Statistical analysis: Includes differentially expressed protein analysis – input will be absolute quantification value for Target 48 or NPX value for Olink® Target 96

SERVICESSample Requirements

Panel Selection Sample Type Tube Type Minimum amount per sample Minimum Sample Number
Target 48

&

Target 96

Plasma
(Collected from EDTA blood tube only)
Leak proof sample tube, recommended protein-lobind tube 30µL Olink® is a high-throughput application, so we ask clients to provide enough samples to fill a plate (minimum 88 samples for Target 96 and 40 samples for Target 48) to ensure statistical significance.
Serum Leak proof sample tube, recommended protein-lobind tube 30µL
Whole Blood Red top

SST

EDTA

1 – 5 mL
Please contact us for consultation and further details.

SERVICESLiquid chromatography–mass spectrometry (LCMS)

LCMS targeted assay

Tool for novel biomarker target

Post-translational ready for lipidomics, glycomics, metabolomics studies.

  • Target (predetermined) peptide molecule with precursor mass, fragmentation info, retention time
  • Precisely select precursor ions of interest and isolate specific fragment ion
  • Peak integration with high speed
  • High multiplexing in small sample volumes
  • Multi-omics integration
  • Scalable from discovery to clinical implementation
Please contact us for consultation and further details.

SERVICESPEA Kits

Please contact us for consultation and further details.